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Acknowledgments: The authors thank Drs. Alan Fogelman and Jerome Rotter for their critical review of this manuscript. Grant Support: By National Institutes of Health grants HL33177 and.
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Additional epithelial specific protein used for detection, we transplanted WT CFTR + ; male GFP + BM into newborn CFTR mice 3 male and 6 female ; . Using in vivo and in vitro CFTR functional assays, we showed previously that marrow derived epithelial cells express functional CFTR after BMT of adult CFTR mice [5]. We now tested whether transplantation into newborn CFTR mice also restored functional CFTR channel activity. In initial experiments, CFTR mice were transplanted after Bu TBI. However, the CFTR- pups did not survive with this regimen data not shown ; . Therefore, in subsequent studies, CFTR- mice n 9 ; were myelosuppressed with the Bu Bu protocol and transplanted on postnatal day 1. These CFTR mice were monitored by in vivo rectal potential difference RPD ; at 12 and 24 weeks post-transplantation Fig 2A, Table 4 ; . Untransplanted CFTR + - mice n 14 ; , which have very similar electrophysiology to wild-type mice [22, 27]; and untransplanted CFTR n 15 ; mice were used as positive and negative controls; respectively. CFTR activity was measured as the change ; in RPD in response to exposure to forskolin [5]. Forskolin induces a hyperpolarization 5.4 1.0 mV in CFTR + - mice and causes a depolarization the PD becomes more positive ; of 3.0 0.5 mV in CFTR animals Fig. 2A, Table 4 ; . This forskolin-induced depolarization in CFTR- mice is due other cAMP responsive channels and the lack of functional CFTR in the mucosa of the distal colon and clonidine, for instance, climara medication.
Student trainers provided a reliable 24 hours of contact time per week with shelter dogs and with potential adopters. This was a very effective way of providing behavioural enrichment for the shelter dogs while educating the public. From April 2003 through March 2004, the PEIHS received a total of 822 dogs. Of these, 164 were reclaimed by their owners, and 296 were adopted out to new homes. Among the dogs who were adopted, only those that spent less than two days on the adoption floor did not receive the benefit of contact from the student trainers. Most of the dogs on the adoption floor remain in the shelter for a period of one or more weeks. Without the presence of the student trainers, these dogs would have very little access to activities outside their runs, and would not be conditioned to show more socially acceptable behaviour. Of the 361 dogs who were deemed to be unsuitable for adoption, 72% were euthanized for behavioural reasons, demonstrating the impact of behaviour problems on the welfare of dogs. Student trainers answer general behaviour questions received by the shelter, and provide ongoing training support to owners of newly adopted dogs. The programme continues to enjoy enormous support from the shelter administration and staff, and, although not everyone continues to clicker train his or her dog after adoption, there is a noticeable increase in the public's awareness of the effectiveness of non-coercive methods of training. In addition to the obvious direct benefits to the shelter dogs, this programme helps to improve the public's perception of the PEIHS and its activities, and can be expected to indirectly foster better attitudes towards animals. Perhaps the most significant impact this programme has on the welfare of animals is the dissemination of knowledge by graduating AVC veterinarians to their colleagues and clients. Although this effect will always be difficult to measure, a graduate's hands-on experience and "comfort" with managing problem behaviour will undoubtedly work to keep many dogs in their homes who might otherwise be relinquished to shelters. Funding for this project has been renewed through 2006. More information can be found at upei ~traindog, and Dr. Guy is also preparing an article on the programme for peerreviewed publication. AVC humane education programme 200204 ; N Guy This programme was begun in 1997 by Dr. Karen Gibson. The objectives over the last two years were to continue the classroom visits, including distribution of learning aids; evaluate the programme through examination of teacher surveys; create a written compilation of student experiences in the classroom for the benefit of new student volunteers; revise the lesson plan book if required; and prepare a manuscript describing the programme for submission to a peer-reviewed journal. The AVC humane education programme continues to be evaluated very highly by those teachers making use of it, and veterinary students encounter few problems in the classroom. However, although the programme has been in place for a number of years and is well-publicized to the schools with annual mailouts, many teachers remain unaware of its existence. In an effort to improve the visibility to teachers, two initiatives were undertaken in the fall of 2003. The first was a fax sent directly to.
Suitable cell line was selected for the determination of CD50 of andrographolide. Three different cell lines, BHK21, MDBK and VERO cell lines were used in this study. Results: To calculated CD50 of andrographolide was 378.8, 460 and 160 mg on BHK21, MDBK and VERO cell lines, respectively. The linear regression analysis of in vitro concentration of andrographolide on per cent cytotoxicity gave the correlation coefficient of 0.88, 0.96 and 0.69, respectively for BHK21, MDBK and VERO cell lines. Conclusion: Thus, the analysis indicated that MDBK cell line could be the most suitable cell line for the CD50 determination of andrographolide and the calculated CD50 for this compound by this method was 460 mg ml. Key words: Andrographolide, cytotoxicity, VERO, BHK21, MDBK 93. A PHARMACOLOGICAL OBSERVATION RAPHANUS SATIVUS LINN RADISH ; SHASHIKALA G.H., ACHARYA S.R.K. Department of Pharmacology, J.J.M. Medical College, Davangere, Karnataka State Objective: Raphanus sativus, the most common vegetable is credited with may therapeutic uses in Ayurveda. No pharmacological studies have yet been undertaken. Hence the present work. Methods: Aqueous extract of radish was evaluated for its action on isolated, superfused rectus abdominis muscle of frog, dorsal muscle of leech, biventer cervicis of chick, guinea pig ileum, rat uterus and perfused isolated frog heart. Results: At 1 mg concentration the extract potentiated the action acetylcholine on skeletal and smooth muscle preparation. At 10 mg and above it produced contractions per se of both skeletal and smooth muscle preparations, which were partially inhibited by pancuronium and atropine respectively. Isolated frog heart was inhibited by the extract, which was partially countered by atropine. Conclusion: Radish, which is credited with many actions and uses, is found to be having an active principle that stimulates both skeletal and smooth muscles. 94. A PHARMACOLOGICAL STUDY OF THE AQ UEOUS EXTRACT OF BANANA SKIN MUSA PARADISICA ; ON ISOLATED PREPARATIONS * SARALA N., ACHARYA S.R.K. Department of Pharmacology, J.J.M. Medical College, Davangere, Karnataka. * Department of Pharmacology, S.D.U. Medical College, Kolar, Karnataka. Objective: Banana and its skin are used in folklore and Ayurvedic medicine as laxative, poultice, anthelmintic, etc. There are no pharmacological studies especially on the skin of banana. Hence the present study. Methods: Aqueous extract of banana skin was studied for its effect on isolated, superfused rectus abdominis muscle and heart of frog, guinea pig ileum and rabbit duodenum. Results: In small concentrations like 100-1000 mcg the extract potentiated acetylcholine. In 1000, 2000 and 4000 mcg skeletal muscle responded with a graded response that was partially countered by gallamine. Frog heart was inhibited by the extract and this was antagonized by atropine. Guinea pig ileum and rabbit ON and combivent.
Patient group: One hundred ten consecutive patients who underwent percutaneous coronary intervention for a right coronary ostial stenosis between July 1990 and October 1995 constituted the study group. Medical charts of all the patients were reviewed for demographic and clinical characteristics. Technical details of the procedures were recorded from the charts as well as the procedure reports. A significant ostial lesion was defined as a lesion with 50% diameter stenosis within 3 mm of the aortic orifice. None of the patients had known syphilitic or inflammatory aorto-arteritis and none had undergone radiation therapy for mediastinal neoplasm. Patients were divided into 3 groups. Group I included 26 patients treated with conventional balloon angioplasty. Group II was composed of 26 patients primarily treated with a debulking device that included excimer laser and directional or rotational atherectomy. Group III was composed of 58 patients treated with intracoronary stents, either primarily or following suboptimal results after balloon angioplasty. The demographic and clinical characteristics of these groups are shown in Table I.
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Neuman MG, Malkiewicz IM, Shear NH: A novel lymphocyte toxicity assay to assess drug hypersensitivity syndromes. Clin Biochem. 33: 517-524 2000 ; . Neuman MG: Role of bile acids blocking ethanol-induced apoptosis. In: Leutchner U, ed. ; . Bile Acids and Liver Disease. Kluwer Academic Publishing, London, UK, pp. 276-295 2000.
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Four potential solutions to the patient medication-safety dilemma are described here: 1. The Academy of Managed Care Pharmacy has developed and published Guiding Principles for Effective Electronic Messaging amcp ; . As these principles become more widely adopted, they should significantly increase the value of safety alerts to pharmacists. Such standardized messages will be clearer, more action-focused, and less redundant than current alerts.38 These same enhancements will also benefit physicians who prescribe electronically. 2. The USP, among its many vital services, has formed a Therapeutic Decision Making Expert Committee to identify those drugdrug interactions for which the risk of harm is the greatest. The USP is evaluating the level of evidence connecting specific drug alerts with an eventual impact on health outcome. At the end of this process, the USP plans to issue recommendations regarding the appropriateness of including or excluding specific drugdrug interactions in a drug review safety program.39 3. Senior Outpatient Safety "SOS Rx" ; , a broad collaborative coalition, has been convened by the National Consumer's League under an unrestricted grant from Express Scripts.40 Representing consumers, caregivers, government agencies, retailers, health plans, pharmacy benefit managers, manufacturers, and professional societies, this group is focusing on advocacy for e-prescribing, personal medication records, education of highrisk patients, and the development of a clearinghouse of information about best practices for high-risk situations. 4. The Medicare Modernization Act of 2003 has introduced a number of initiatives for improving patient safety: Pharmacy transactions will have to include both concurrent and retrospective DURs with associated safety interventions. The adoption of e-prescribing will be accelerated. An intervention called Medication Therapy Management Services will be implemented to improve selfmanagement of complex medication regimens and to aid in detecting ADRs, for instance, climqra transdermal patch.
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Biopsies at 6 months. In our study, biopsies were also evaluated by means of a morphometric technique in order more accurately to test a possible association between RI and structural damage. We evaluated Vg, Vvint cortex and Vvintima artery because they are surrogate measures of CAN [7, 11]. However, none of these parameters was associated with the RI, which indicated that RI cannot be considered as a surrogate measure of chronic structural damage, at least during the first few months of follow-up. In the present study, we confirmed that patients displaying CAN in the protocol biopsy were at increased risk for graft function deterioration [5, 6], whereas we were unable to find any association between RI and graft outcome. This result supports the concept that histology is superior to RI for predicting graft function deterioration, at least in patients with stable renal function. In contrast, Pape et al. [12] reported an association between RI and chronic damage evaluated in sirius red-stained biopsies in deteriorating paediatric renal allografts. In the current study, both RI and chronic damage were independent predictors of graft function deterioration. However, it should be recalled that RI in adult populations is strongly influenced by aortic compliance, but this is probably not the case in children. Thus, we speculate that in children, the association between RI and renal structural damage may be more intense than in adults since there is less interference by aortic stiffness. Although RI has been employed to evaluate renal transplant dysfunction, it has been shown to have a low specificity and sensitivity for the diagnosis of acute or chronic dysfunction [13]. Despite the low utility of RI as diagnostic tool, it recently has been shown to be a powerful predictor of late graft failure [4]. This discrepancy between the diagnostic and prognostic utility of RI raises questions about its physiological significance. It has been proposed that RI provides a surrogate measure of renal vascular resistance; however, experimental and clinical data indicate that this parameter is better related to vascular compliance [10, 14]. In an isolated perfused kidney model, the physiological parameter that most closely correlates with increased RI is an increased pulse pressure index. This correlation explains the observed associations between age and RI in the general population, and the association between recipient age and RI but not that of donor age in transplanted patients [9]. In agreement with Radermacher et al. [4], we observed a negative association between CsA levels and RI. Since CsA treatment has been associated with various cardiovascular changes, such as vasoconstriction and tachycardia, further studies will be needed to characterize the relationship between these two variables. We observed an association between RI and glomerulitis, but not between RI and interstitial infiltration, tubulitis or vasculitis. The evaluation of biopsies according to Banff criteria is associated with a significant intra- and inter-observer variability [15].
DISCLOSURES This article is based on the proceedings of a Foundation for Managed Care Pharmacy symposium held on July 17, 2004, in Napa Valley, California, and supported by an educational grant from TAP Pharmaceutical Products, Inc. The author did not receive an honorarium for participation in the symposium upon which this article is based; he discloses no potential bias or conflict of interest relating to this article. REFERENCES 1. Academy of Managed Care Pharmacy. AMCP's Framework for Quality Drug Therapy. Available at: : fmcpnet fmcp ?c news&t detail&id 57. Accessed December 2004. 2. Kohn LT, Corrigan JM, Donaldson MS, eds. To Err Is Human: Building a Safer Health System. Institute of Medicine Committee on Quality of Health Care in America. Washington, DC: National Academy Press; 2000. Available at: : iom report ?id 5575. Accessed November 13, 2004. 3. Schuster MA, McGlynn EA, Brook RH. How good is the quality of health care in the United States? Milbank Q. 1998; 76: 517-63, Kohn LT, Corrigan JM, Donaldson MS, eds. Crossing the Quality Chasm: A New Health System for the 21st Century. Institute of Medicine Committee on Quality of Health Care in America. Washington, DC: National Academy Press, 2001. Available at: : iom report ?id 5432. Accessed November 13, 2004. 5. Kotter JP. A Force for Change: How Leadership Differs From Management. New York, NY: The Free Press; 1990. 6. Kotter JP Leading Change. Boston, MA: Harvard Business School Press; 1996 7. Covey SR. The 7 Habits of Highly Effective People. 1st ed. Chicago, IL: Thorndike Press; 1990. 8. Rogers EM. Diffusion of Innovations. 4th ed. New York, NY: The Free Press; 1995.
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