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2nd Department of Pediatrics, University Hospital, Brno Introduction Microscopic, lymphocytic, collagenous colitis are well-defined entities with typical pathological findings in large bowel mucosa. Corresponding clinical symptoms are much less stringently defined and their relation to other autoimmune disorders is not yet fully understood. Methods In a cohort of 12 patients aged 15 4.5 years we performed colonoscopy because of chronic diarrhoea 9 12 ; abdominal pain with upper dyspepsia 4 12 ; , loss of appetite 6 12 ; , weight loss 3 12 ; , irritable bowel according to Rome II for pediatric patients and GSRS 10 12 ; . Sampling of mucosa was taken at 20, 60, and 90 cm respectively. We performed screening for celiac disease with antibodies anti-gliadin IgG, reticulin, endomysium and transglutaminase IgG and IgA and genotyping PCR SSR Dr3, 5, 7 and also, autoimmune screening with IBD, ASCA pANCA and ultrasonic examination of terminal ileum was done. Next, we performed enterobiopsy using Crosby capsula from the first part of jejunum. In both negative and positive patients, we followed compliance with dietary measures over 6 months using GSRS and serology. Results In this group we diagnosed celiac disease in four patients 33.3 % ; . In no subject did we demonstrate the presence of classic total atrophy or Marsh 3 and more. In one patient we observed minimum microscopic infiltrative changes with IEL 100 cells 50 and or more and in the remaining, the picture corresponding to Marsh 3 was present. In the whole cohort, increase of anti-gliadin and anti-transglutaminase IgG was present. Moreover, in one patient with Marsh 3, positive EMA IgA was also observed. In all patients the presence of either mutation in DrHLADq was shown. Regarding compliance, no difference was observed between the patients with minimum lesions and those with fully developed celiac disease within 3 months, Z score was p 0.05 after 6 months using dietary treatment only. In both groups, diet led to clinical improvement. Conclusion Mild coeliac disease with DQ positive and EMA negative is probably more frequent in patients with minimal colitis than hitherto thought. The problem may lie in the mode of diagnosis of celiac disease. Minimum differences in the influence of dietary measures consisting of elimination of gliadin can be caused either by the presence of non-HLA positive celiac patients in the group of minimum colitis patients or by some unspecific influence of diet on the course of and atrovent and altace, for example, altace rash!

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